Cryoprocess

This is the freeze-dry method, where the creature is frozen immediately upon killing or death and left frozen for a long period of time, during which time it is completely desiccated by the cryoprocess. If this method is used, it may be necessary to relax the specimen or specimens overnight unless the specimens were pinned and legs arranged for the cryoprocess, which all depends upon the space available in the freezer.

The course of the cryoprocess in the temporal-bone specimens was studied partly by measuring the course of the temperature in the lateral semicircular canal and in the facial canal by thermocouples, partly by measuring the heat flow and total amount of heat exchanged.

Because of the extensive investigations underway with this procedure, further discussions on this cryoprocess of macromolecule separation follow.

Application of thermodynamics to cryopreservation is expected to improve the conservation of germplasm. The goal is to run the cryoprocesses under well-defined rules based on the latest scientific achievements in the field of low-temperature thermodynamics.

The cost of the cryoprocess gets reduced when milling operations are carried out on a bigger scale and with efficient recycling of the refrigerant. The maxiumu yield of oil has been obtained when the cardamom seeds are precooled by using liquid nitrogen within a temperature range of –180 °C to –190 °C and grinding the seeds to a size of 250 μ, which is, indeed, a fine powder size.

Work on several yeasts has shown preservation of cell surface structures by cryoprocessing that had not been possible with conventional procedures (Baba and Osumi 1987; Käppeli et al. 1984; Walther et al. 1984; Tokunaga et al. 1986).

A third porition was fixed in 0.25% gluteraldehyde, cryoprocessed, sliced, and stained with antisera to progesterone previously adsorbed with steroid extracted placental powder.