Lickspout

To quantify the percentage of AgRP, ARC inh, and ARC other neurons modulated by placement of the lickspout or feeding, we compared the distribution of firing rates (estimated in 5-s bins) before lickspout placement (up to 5 min prior to lickspout placement) with the distribution of firing rates post-lickspout placement (‘food cue predictive responses’; up to 5 min post lickspout, but only including time bins prior to food availability; Figure 4 ) or post-Ensure delivery (early-feeding responses: 0–5 min following onset of Ensure availability; mid-feeding response: 5–15 min following onset of Ensure availability; late-feeding response: 15–45 min following onset of Ensure availability; Figure 3 ) via a two-sample KS-test ( Figure 3E ; p < 0.025).